July 2019, Vol. 4, No. 7, pp 186-191.
Use of Mucor strictus, Rhizomucor Michei and Saccaharomyces cerevisiae for the Detoxification of Cyanide Gotten from Cassava Products through Solid State Fermentation
Amos Stephen¹, Inalegwu Bawa¹, Ogo Ogo², Ogli Itolo¹
¹Department of Biochemistry, Federal University of Agriculture, Makurdi, Benue State, Nigeria.
²Department of Biochemistry, Benue State University, Makurdi, Benue State, Nigeria.
*Corresponding author’s e-mail: stephenamos04@gmail.com
Abstract
Microbial fermentation has been reported as an effective means of cyanogens removal of cassava products. The research is aimed at using three species of Fungi (Mucor strictus, Rhizomucor michei andSaccharomyces cerevisae) for the detoxification of cyanide through solid state fermentation. The results of this study depict the following; the cyanide contents of the cassava leave, tubers and peels are 2.025±0.9, 0.76±0.01 and 2.00±0.08 respectively. After 96 hours of all the three isolated fungi, fermentation time has the best reducing effect on the cyanide content of the cassava samples at varying significance (P>0.05) across the groups. The study depicts that Rhizor M. had the highest activity after 96 hours in reducing the cyanide content of the cassava tubers and peels where it significantly reduced it (P>0.05) from 0.76±0.01, 2.0±0.07 to 0.17±0.05 and 0.17±0.50 respectively. M. strictus had the highest reducing activity on the cassava leaves where it significantly reduced (P>0.05) the cyanide content from 2.00±0.02 to 1.40±0.02. To existing knowledge, this study has contributed that: S. cerevisiae and R. mucor have the best cyanide reducing activity on the cassava peels, M. strictus has the best reducing activity of cyanide on the cassava leaves and R. mucor had the best activity in reducing cyanide contents of the tubers.
Keywords: Mucor strictus; Rhizormucor michei; Saccharomyces cerevisiae; Detoxification; Cyanide; Cassava; Fermentation.
References
- Biraj D, Siddharth VS, Shoeb A, Abhineet Adeleke BS, Akinyele BJ, Olaniyi OO, Jeff-Agboola YA. Effect of Fermentation on Chemical Composition of Cassava Peels. Asian Journal of Plant Science and Research 2017;7:31-8.
- Bolhius GG. The toxicity of cassava roots. Netherlands Journal of Agricultural Science 1954;2:176-85.
- Conn EE. Cyanogenic glucosides. Journal of Agriculture Food and Chemistry 1969; 17:519-26.
- Cooke RD, Maduagwe EN. The effects of simple processing on the cyanide content of cassava chips. Journal of Food Technolnology 1978;13:299-306.
- De Bruijn GH. The cyanogenic character of cassava (manihot esculenta in chronic cassava toxicity). Proc. Interdisciplinary workshop London. 1973;43-48
- Eustace A, Dorothy M. Reducing toxicity by simple changes in the traditional preparation of cassava leaves- a small trial in Mozambique. Journal of Tropical Science 2000;29:269-72.
- Fakir MSA, Mostafa MG, Seal HP. Food security in Bangladesh: Selection, nutritional status evaluation of processing technique of cassava strains for use as a potential human and animal food. Poster presented ‘In’ Intl. Conf. “Food security during challenging times” Univ. Putra Malaysia, Selangor, Malaysia 2009;18-220.
- Fakir MSA, Mostafa MG, Seal HP. Food security in Bangladesh: Evaluation of cassava (Manihot esculenta) morphotypes based on hydrogen cyanide acid toxicity and protein content of tuber. Poster presented In Natl. Symp. Climate change, plant protection & food security interface, Assoc. Advan. Plant protection, BCKV, Kalyani, India, 2010;59:17-19:
- Iyayi EA, Tewe OO. Cassava feeding in small holder livestock units. Acta Journal of Horticulture 1994;375:261-70.
- Komolafe ME, Adegbola AA. Agric. Science second edition. Food Chemistry. 1981;94.
- Montagnac JA, Christopher RD, Tanumi SA. Processing technique to reduce toxicity and antinutrients of cassava for use as staple food. Comprehensive Revised Food Science Food Safety 2009;8:17-27
- Oke OL. Processing and detoxification of cassava. Journal of Acta Horticulture 1983; 82:599-602.
- Oke OL. Eliminating cyanogens from cassava through processing: Technology and tradition. Journal of Acta Horticulture 1994;375:163-74.
- Oki TR. The use of cassava leaf as plant protein source for broiler production. Ph.D. Thesis, University of Nigeria Nsukka. 1998.
- Prince NR. The mode and action of fumigants. Journal of Stored Products Research 1985;21:157-164.
- Tewe OO. Implications of the cyanogenic glucoside fractions of cassava in the growth and reproductive performance of rats and pigs. Ph.D. Thesis, University of Ibadan, Ibadan, 1975.
- Montagnac JAC, Davis CR, Tanumihardjo SA. Nutritional value of cassava for use as a staple food and recent advances for improvement. Comprehensive review food science. Food Safety 2009;8:181-94
- Ojo A, Akande EA. Quality evaluation of “gari’ produced from cassava and potato tuber mixes. African Journal of Biotechnology 2013;12:4920-24.
- Monday AO, Simon TU, Agbajor K. Proximate Analysis and Mineral Composition of Peels of Three Sweet Cassava Cultivars. Asian Journal of Physical and Chemical Sciences 201.;34: 1-10.
- Olufunke OE, Ogugua CA. Solid State Fermentation of Cassava Peel with Trichoderma viride (ATCC 36316) for Protein Enrichment World Academy of Science, Engineering and Technology. International Journal of Nutrition and Food Engineering 2013;3:22-3.
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