ISSN 2456-0235

International Journal of Modern Science and Technology

INDEXED IN 

​​​​​​​​​​​​​May 2018, Vol. 3, No. 5, pp 112-116. 

​​​Isolation of Coelomic Fluid from Earthworm and its Antibaterial Activity

N. Prabhu*, K. Divya, R. Keerthana, S. Prithivi, M. Suganya
Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode, Namakkal, Tamilnadu. India.
​​​*Corresponding author’s e-mail: prabhu.aut.26@gmail.com

​Abstract

​In naturally, earthworms were secreted the coelomic fluids which helps in the movement of earthworm and also it consist of many proteins which are responsible for microbial resistant from environment. The present work that the coelomic fluids were collected using cold shock method and were separated from debris by centrifugation method and the isolated coelomic fluid was tested the concentration of protein presence by Bradford’s method and the proteins are analyzed using SDS-PAGE. Then the antimicrobial activity was done through the agar well diffusion technique with different concentrations of crude coelomic fluids. The result revealed that the antibacterial activity in earthworm shows that the zone of inhibition of coelomic fluid against Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The present work conclude that the Eudrilus eugeniae coelomic fluid shows antibacterial properties against selected bacterial isolates and suggests some of the coelomic fluid components might be useful for pharmaceutical applications in future.

Keywords: Coelomic fluid; SDS-PAGE; Bradford; Anti-bacterial activity.

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