ISSN 2456-0235

International Journal of Modern Science and Technology

INDEXED IN 

​​​​​​​​​​​​​October 2018, Vol. 3, No. 10, pp. 208-212. 

​​​Diabetes Induction with Streptozotocin and Insulin Action on Blood Glucose Levels in Albino Rats

J. A. Jato*, I. Bawa, F. N. Onyezili
Department of Biochemistry, University of Agriculture Makurdi, P.M.B 2373, (970001) Makurdi, Nigeria.

​​​*Corresponding author’s e-mail: jatojack@gmail.com

​Abstract

​The present study was designed to investigate diabetes induction with streptozotocin (STZ) in albino rats and the effect of insulin on the blood glucose levels of rats induced with diabetes. Fasting blood sugar (FBS) and oral glucose tolerance test (OGTT) was used to determine glucose levels in experimental groups for 14 days. The results of FBS on days 0, 7 and 14 show that rats treated with 4 μl/kg of body weight daily maintain normal glucose levels (99.50±3.51, 95.83±5.19 and 96.83±5.04) as those which were not induced with diabetes (89.33±5.32, 89.17±4.26 and 89.17±5.19), while the none treated rats were h diabetic all through (375.33±39.53, 381.67±35.44 and 375.67±36.74). OGTT at day 14 showed initial rise in glucose levels at 0 min for non-diabetic and insulin treated diabetic rats after administration of 2 g/kg body weight load of glucose, at 60-120 min normal levels were maintained (100.67±9.1, 117.00±19.0, 95.83±7.3 and 91.83±9.1, 101.00±13.4, 91.67±10.0) respectively. Conversely non treated rats were unable to achieve normal levels (287.00±19.8, 360.83±40.8 and 293.50±13.5). This finding may be helpful in diabetes research that has to do with regulation and maintenance of glucose levels.

Keywords: Diabetes; Insulin; Glucose; Strptozotocin. 

​References

  1. ​​American Diabetes Association (2013) Diagnosis and Classification of Diabetes Mellitus. Diabetes Care, 36, Supplt 1 (care.diabetesjournals.org).
  2. Masharani U, German MS. Pancreatic Hormones and Diabetes mellitus. In: Gardner DG, Shoback D. (eds.) Greenspan's basic & clinical endocrinology 9th ed. New York: McGraw- Hill Medical; 2011. p. 605 Chapter 17.
  3. IDF Diabetes Atlas. Fifth Edition 2011.
  4. Arowosegbe S, Olanipekun MK, Kayode J. Ethnobotanical Survey of Medicinal Plants used for the Treatment of Diabetes Mellitus in Ekiti South Senatorial District, Nigeria.  European Journal of Botany, Plant Sciences and Phytology 2015; 2(4): 1-8,
  5. Carvalho E, Moura J, Børsheim E. The Role of MicroRNAs in Diabetic Complications—Special Emphasis on Wound Healing. Genes 2014; 5: 926-956; doi:10.3390/genes5040926
  6. Kimani CN, Mbaria JM, Suleiman M, Gakuya D, Kiama SG. Antihyperglycemic activity of Zanthoxylum chalybeum stem-bark extract in diabetic rats. Journal of phytopharmacology. 2015; 4(3): 183-189
  7. Chinenye S, Young E. State of Diabetes Care in Nigeria: A Review. The Nigerian Health Journal, 2011; 11(4): 101-106
  8. Yamany AA, Sayed HM. Effect of low level laser therapy on neurovascular function of diabetic peripheral neuropathy Journal of Advanced Research 2012; 3, 21–28
  9. Forbes JM, Cooper ME. (2013) Mechanisms of Diabetic Complications. Physiol Rev 2013; 93: 137–188.
  10. Usman UK, Abubakar AB, Mohamed M. A Review on Experimental Methods of Diabetic Research: Advantages and Limitations. Annual Research & Review in Biology 2015; 7(2): 100-108
  11. Mendes JJ, Leandro CI, Bonaparte DP, Pinto AL. A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies American Association of Laboratory Animal Sciences 2012; 62(1): 37–48
  12. Islam S, Loots DT. Experimental Rodent Models of Type 2 Diabetes: A Review. Methods and Findings in Experimental and Clinical Pharmacology 2009; 31(3): 1-14.
  13. Institute for Laboratory Animal Research. Guide for care and use of Laboratory Animal. Washington (DC): National Academies Press [PubMed]. 1996
  14. Kaliwal  BB, Shetti AA, Sanakal  RD. Antidiabetic effect of ethanolic leaf extract of Phyllanthus amarus in alloxan induced diabetic mice. Asian Journal of Plant Science and Research, 2012; 2(1): 11-15
  15. Gupta R, Sharma AK. Anti-Hyperglycemic Activity of Aqueous Extracts of Some Medicinal Plants on Wistar Rats. Journal of Diabetes and Metabolism. 2017; 8: 752. doi:10.4172/2155-6156.1000752
  16. Eleazu CO, Eleazu KC, Chukwuma S, Essien UN. Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. Journal of Diabetes & Metabolic Disorders 2013; 12:60: 1-7.
  17. Busineni JG, Dwarakanath V, Chikka swamy BK. Streptozotocin- A Diabetogenic Agent in Animal Models. International Journal of Pharmacy and Pharmaceutical Research; 2015; 3(1): 253-269
  18. Okoro IO, Umar IA, Atawodi SE, Anigo KM. Comparative Antihyperglycemic Effect of Petroleum Ether, Acetone, Ethanol and Aqueous Extracts of Cleome rutidosperma DC and Senecio biafrae (Oliv. and Hiern) in Streptozotocin-induced Diabetic Mice British Journal of Pharmacology and Toxicology 2014;5(3):115-24.
  19. Chaimum-aom A, Chomko S, and Talubmook C. Toxicology and Oral Glucose Tolerance Test (OGTT) of Thai Medicinal Plant Used for Diabetes controls, Phyllanthus acidus L. (EUPHORBIACEAE). Pharmacog J 2017;9(1):58-61
  20. Ünal D, Kara A, Aksak S, Altunkaynak BZ, Yıldırım S. Insulin hormone: Mechanism and effects on the body and relationship with central nervous system. Dicle Medical J  2012;39 (2): 310-15.