ISSN 2456-0235

International Journal of Modern Science and Technology

INDEXED IN 

​​​​​​​​International Journal of Modern Science and Technology, Vol. 2, No. 3, 2017, Pages 98-104. 

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​Anticancer Activity of Iron oxide Nanobiocomposite of Fungal Asparaginase

G. Baskar, J. Chandhuru, A. S. Praveen, K. Sheraz Fahad
​Department of Biotechnology, St. Joseph’s College of Engineering, Chennai – 600 119. India. 
*Corresponding author’s e-mail: basg2004@gmail.com

​Abstract
Drug delivery using nanoparticles has been found to be more effective compared to normal drug delivery and has many advantages. Iron oxide nanoparticles synthesis is of greater importance because targeting the drug to the desired location is brought by applying magnetic field, which is of lesser cost. In the present work iron oxide nanobiocomposite was produced using L-asparaginase rich culture filtrate produced from Aspergillus terreus. Synthesized iron oxide nanobiocomposite was characterized using UV-Vis spectrum, X-Ray Diffraction (XRD), Fourier Transform Infra-Red (FTIR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray (EDX) analysis. An absorption peak at 330 nm confirmed the formation of iron oxide nanoparticles by fungal culture filtrate with asparaginase. The presence of functional groups like O-H, C=O, C-H and C=N on surface of the iron oxide nanobiocomposite was confirmed by FT-IR analysis. The XRD analysis proved that the synthesized nanobiocomposites are crystalline in nature. Produced nanobiocomposites were in the size range of 40-100 nm. The EDX analysis confirmed the presence of iron and oxygen in the synthesized nanobiocomposite. The MTT assay was carried to study the Anti-cancerous nature of the synthesized nanobiocomposites on MCF-7 cell line.  MTT assay showed that decrease in cell viability, the viability of the cells decreased to 41.9% when it was treated with iron oxide nanobiocomposite of asparaginase. Thus it is proved that the iron oxide nanobiocomposite of asparaginase is a potential anticancer drug. 

​​Keywords: Nanobiocomposite; Characterization; L-asparaginase; Anticancer activity; Drug delivery.

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ISSN 2456-0235