ISSN 2456-0235

International Journal of Modern Science and Technology

INDEXED IN 

​​​​​Vol. 2, No. 12, 2017, pp. 397-403. 

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​Immobilization of Cellulase on Cobalt Oxide Nanoparticles for Efficient Bioethanol Production by Simultaneous Saccharification and Fermentation

Elsa Cherian¹,*, M. Dharmedira Kumar², G. Baskar¹, R. Kalpana¹
¹Department of Biotechnology, St. Joseph’s College of Engineering, Chennai - 600119. India.
²Department of Applied Science and Technology, A. C. Technology, Anna University, Chennai - 600025. India. 
​​​*Corresponding author’s e-mail: elschk@gmail.com

​Abstract

​Cellulose is a major biopolymer found in plants which can be used for many industrial applications. Major portion of cellulose is getting wasted along with forest and agricultural waste. This can be efficiently used for the production of many industrially important products. Availability of limited fuel, which is a major threat in the present scenario, can be solved upto an extent by the production of biofuel. In the present study cellulase produced by of Aspergillus fumigatus JCF was used for the bioconversion of agricultural wastes to bioethanol. Produced cellulase was immobilized on cobalt oxide nanoparticles for increased catalytic efficiency and utility. Immobilized cellulase was found to retain 75% of the activity even after 4th cycle. Pretreated miscanthus leaves were used as substrate for bioethanol production by simultaneous saccharification and fermentation using immobilized and free cellulose and baker’s yeast. Action of immobilized cellulaseand baker’s yeast released more bioethanol of about 21 g/l which was higher than free cellulase. Thus the immobilized cellulase was found more effective than free cellulase for hydrolysis of agricultural waste to produce of bioethanol.

Keywords: Aspergillus fumigatus JCF; Cellulase; Immobilization; Cobalt oxide nanoparticles; Bioethanol; Simultaneous Saccharification and fermentation.

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