International Journal of Modern Science and Technology

INDEXED IN 

ISSN 2456-0235

​​​International Journal of Modern Science and Technology, 1(8), 2016, Pages 288-295. 


Wound Healing Ethano-pharmacological Potential of Anisomeles malabarica 

G. Baskar, J. Chandhuru, A. S. Praveen, K. Sheraz Fahad
Department of Biotechnology, St. Joseph’s College of Engineering,Chennai – 600 119. India.

Abstract
Nanotechnology has made it possible to coat therapeutic agents on the nanoparticles.  Nanoparticles are used as drug carrier to deliver anticancer therapeutic agents to cancer cells for increased bioavailability. Fungal L-asparaginase is a potential anticancer agent with lesser side effect. In the present study, L-asparaginase synthesized by Aspergillus terreus was conjugated with gold nanoparticles to form gold nanobiocomposite of asparaginase. The formation of gold nanobiocomposite was confirmed by absorption peak at 540 nm in UV-Visible spectrometer. The crystalline nature of synthesized gold nanobiocomposite was confirmed using XRD analysis. Both spherical shaped needle shaped gold nanobiocomposites were observed in SEM analysis with the size ranges between 150-250 nm and 60-80 nm respectively. The MTT assay showed that decrease in viability of MCF-7 cancer cell line. The cell viability was decreased to 36.74% when MCF-7 cancer cells treated with gold nanobiocomposite of asparaginase. Thus the synthesized gold nanoparticles conjugated with asparaginase can be used as bi-functional nanomaterials for cancer diagnosis and treatment.

​​Keywords: Gold nanoparticles; Nanobiomaterials; Characterization; L-Asparaginase; Anticancer activity. 

​References

  1. Kaittanis C, Shaffer TM, Daniel LJ, Thorek DLJ, Grimm J. Dawn of Advanced Molecular Medicine: Nanotechnological Advancements in Cancer Imaging and Therapy. Crit Rev Oncog. 2014;19:143-176.
  2. Astruc D. Introduction to Nanomedicine. Molecules. 2016;21(1):4.
  3. Boisselier E, Astruc D. Gold nanoparticles in nanomedicine: preparation, imaging, diagnostics, therapies and toxicity. Chem Soc Rev. 2009;38:1759-1782.
  4. Llevot A, Astruc D. Application of gold nanoparticles to the diagnostic and therapy of cancer. Chem Soc Rev. 2012;41:242-257.
  5. Li X, Xu H, Chen ZS, Chen G. Biosynthesis of nanoparticles by microorganisms and their applications. Journal of Nanomaterials. 2011:ID 270974.
  6. Patra JM, Panda SS, Dhal NK. A review on green synthesis of gold nanoparticles. Int J Pharm Bio Sci. 62015;6:251-261.
  7. Husseiny IM, El-Aziz AM, Badr Y, Mahmoud AM. Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spectrochimica Acta Part A. 2007;67:1003-1006.
  8. Radhika Rajasree SR, Suman TY. Extracellular biosynthesis of gold nanoparticles using a gram negative bacterium Pseudomonas fluorescens. Asian Pacific Journal of Tropical Disease. 2012;S:795-799.
  9. Mishra A, Tripathy SK, Wahab R, Jeong SH, Hwang I, Yang YB, Kim YS, Shin HS, Yun S. Microbial synthesis of gold nanoparticles using the fungus Penicillium brevicompactum and their cytotoxic effects against mouse Mayo blast cancer C2C12 cells. Appl Microbial Biotechnol. 2011;92:617-630.
  10. Sheikhloo Z, Salouti M, Katiraee F. Biological Synthesis of Gold Nanoparticles by Fungus Epicoccum nigrum. J Clust Sci. 2011;22:661-665.
  11. Anitha TS, Palanivelu P. Synthesis and Structural Characterization of Polydisperse Silver and Multishaped Gold Nanoparticles Using Fusarium Oxysporum MTCC 284” Digest Journal of Nanomaterials and Biostructures. 2011;6(4):1587-1595
  12. Narayanan KB, Sakthivel N. Facile green synthesis of gold nanostructures by NADPH-dependent enzyme from the extract of Sclerotium rolfsii. Spectrochimica Acta Part A. 2011;380:156–161.
  13. Inbakandana D, Venkatesan R, Ajmal Khan S. Biosynthesis of gold nanoparticles utilizing marine sponge Acanthella elongate. Colloids and Surfaces B: Biointerfaces. 2010;81:634-639
  14. Vijayakumar R, Devi V, Adavallan K, Saranya D. Green synthesis and characterization of gold nanoparticles using extract of anti-tumor potent Crocus sativus. Physica E. 2011;44:665-671.
  15. Aswathy Aromal S, Vidhu VK, Philip D. Green synthesis of well-dispersed gold nanoparticles using Macrotyloma uniflorum. Spectrochimica Acta Part A. 2012;85:99-104.
  16. Broome JD. L-Asparaginase: Discovery and development as a tumor-inhibitory agent. Cancer treatment reports. 1981;65(S4):111–114.
  17. MIM Sarquis, Oliveira EMM, Santos AS, da Costa GL. Production of L-asparaginase by filamentous fungi. Mem Inst Oswaldo Cruz. 2004; 99:489-492
  18. Pal T, Pal A, Panigrahi S. Nanotechnology in Biology and Medicine: Methods, Devices, and Applications (Ed. Tuan Vo-Dinh), CRC Press; Boca Raton, FL: 2006.
  19. Pathak P, Katiyar VK. Multi-Functional Nanoparticles and Their Role in Cancer Drug Delivery – A Review, AZojomo (Journal of Material online). 2007:DOI: 10.2240/azojono0114.
  20. Baskar G, Chandhuru J, Fahad KS, Praveen AS, Chamundeeswari M. Anticancer activity of fungal L-asparaginase conjugated with zinc oxide nanoparticles. Journal of Materials Science: Materials in Medicine. 2015;26:43.doi:10.1007/s10856-015-5380-z.
  21. Baskar G, Renganathan S. Production of L-asparaginase from natural substrates by Aspergillus terreus MTCC 1782: Optimization of carbon source and operating conditions. International Journal of Chemical Reactor Engineering. 2011;9(1): DOI: 10.1515/1542-6580.2479.
  22. Wriston JC Jr, Yellin TO. L-asparaginase: a review. Adv Enzymol Relat Areas Mol Biol. 1973;39:185-248.
  23. Mossman T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63.