International Journal of Modern Science and Technology

INDEXED IN 

ISSN 2456-0235

International Journal of Modern Science and Technology, 1(1), 2016, Pages 34-41. 


Micropropogation of wild Indian medicinal plant Phyllanthus debilis
B. Bharathiraja1, R. Praveenkumar2, S. Chozhavendhan2, J. Vinoth Arulraj2, A. William Johnson2
1Department of Chemical Engineering, Vel Tech High Tech Dr Rangarajan Dr Sakunthala Engineering College, Chennai. India.

2Department of Biotechnology, Arunai Engineering College, Tiruvannamalai. India.

​Abstract
An efficient micropropagation protocol was developed for Phyllanthus debilis (Euphorbiaceae) an important herb mainly known for medicinal purpose. Nodal segments grown on MS medium containing BA (4.44 µM) and NAA (0.14 µM) recorded 75 % shoot regeneration producing highest number of total shoots (8.33 ± 1.15) with an average shoot length of 6.43 ± 0.06 cm after 35 days. Roots were induced after transfer to half strength MS medium supplemented with IBA (2.46 µM) in terms of average number of roots (6.0± 1.0) with mean root length of 4.90 ± 0.26 cm per shoot with 75±5.0 % of rooting response recorded after 30 days of culture .The rooted plantlets were transferred for hardening, 80 % of plants were successfully established in the field. Antioxidant properties of mother plant, in vitro grown plant and callus culture were evaluated using DPPH assays. The methanolic extract from the leaf extract of mother plants and in-vitro grown plants recorded the most effective DPPH radical scavenging activity (70.67±3.06%) with the values being close to synthetic antioxidant (BHT) as positive control. Callus extract of P. debilis was recorded the most effective DPPH radical scavenging activity (56.4 %). These results establish the antioxidant potential of P. debilis, which could be used as natural antioxidant source.

​​Keywords: Phyllanthus debilis; Antioxidants; Scavenging activity; Free radicals; Micropropagation.


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